Journal: Oncogene
Article Title: CPEB1 Mediates Epithelial-to-Mesenchyme Transition and Breast Cancer Metastasis
doi: 10.1038/onc.2015.350
Figure Lengend Snippet: CPEB1 and MMP9 in human breast metastases. (a) CPEB1-depleted MCF7 cells were transfected with 20nM siRNA against MMP9. After 48 hours, the relative levels of MMP9 were determined by western blot. Migration assay (b) and invasion assay (c) following MMP9 knockdown. Forty-nine samples of normal (9), stage IIA–IIB (24), or stage IIIA–IIIC (16) human breast cancer metastatic tissue were immunostained for CPEB1 and MMP9. The ratio of these two proteins was calculated based on fluorescence intensity. (b) Immunostaining of normal, stage IIA and stage IIIC human metastatic breast tissue with antibody for CPEB1 (green) and MMP9 (red). DAPI was used as the counterstain.
Article Snippet: Human breast tissue array slides containing normal, cancer, and metastatic biopsies were purchased from SuperBioChips Laboratories (Korea).
Techniques: Transfection, Western Blot, Migration, Invasion Assay, Knockdown, Fluorescence, Immunostaining